Cation exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Cation exchange chromatography, more specifically, uses a negatively charged ion exchange resin with an affinity for molecules having net positive surface charges.
Besides, how do you elute cation exchange chromatography?
- An impure protein sample is loaded into the ion exchange chromatography column at a particular pH.
- Charged proteins will bind to the oppositely charged functional groups in the resin.
- A salt gradient is used to elute separated proteins.
- Unwanted proteins and impurities are removed by washing the column.
Subsequently, question is, what is the mobile phase in ion exchange chromatography? Mobile phase (Eluent) In ion exchange chromatography generally eluents which consist of an aqueous solution of a suitable salt or mixtures of salts with a small percentage of an organic solvent are used in which most of the ionic compounds are dissolved better than in others in.
Thereof, what is the principle of ion exchange chromatography?
Ion chromatography (or ion–exchange chromatography) is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. In this type of chromatography, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it.
How does a cation exchange work?
Ion exchange is a reversible chemical reaction where dissolved ions are removed from solution and replaced with other ions of the same or similar electrical charge. Not a chemical reactant in and of itself, IX resin is instead a physical medium that facilitates ion exchange reactions.